Blog
Educational material, comparison studies and publications on SPR and other related techniques for researchers.
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Biolayer Interferometry vs Surface Plasmon Resonance Comparison
Deciding which methods your lab should invest in can be challenging. Cost, experiment time and reproducibility are likely the three most important factors during your evaluation process. We understand that funding is more limited now than ever before, so finding a cost-effective, reproducible method is crucial. Furthermore, there are several pros and cons among various […]
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New Publication – OpenSPR Binding Data Enhances Nanotherapeutic Construction with Molecular Affinity Strategy
In order for drugs to fight diseases, an efficient delivery method must be found. This necessitates an important area of study to ensure that drugs can effectively combat diseases. A subsection of this ever-expanding field is nanomedicine; as it’s name implies, nanomedicine straddles the fields of medicine and nanotechnology, in an effort to increase drug […]
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How to Confront the Antibody Reproducibility Crisis using SPR
Have you ever ordered antibodies from an antibody supplier? Have you ever had a problem with antibody specificity, selectivity and reproducibility? Have you ever returned your antibodies back to the supplier only to receive another poor quality sample? If you answered yes to the questions above, you’ve suffered from the pandemic of “bad” antibodies. It’s […]
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The ABC’s of Competitive Binding Assays with SPR
In the arena of drug discovery, there are a number of tools available for researcher’s to characterize binding behaviours of low molecular weight compounds with their target(s). These tools may include fluorescence or radiolabelling assays, keeping in mind that it is always recommended to avoid using labels as these may alter the spin (i.e. this […]
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OpenSPR Binding Data Uncovers Antipsychotic Drug Mechanisms
Over the last number of years, there’s been an increasing amount of attention on combating mental illness and the associated stigma. Notably, psychiatric counseling is an important facet of treatment when dealing with mental illness in all cases. However, in more moderate-to-severe cases, therapeutic intervention may be necessary to overcome challenging symptoms and provide adequate […]
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Technical Guide: Binding Assays with Flow Cytometry and SPR
Flow cytometers are excellent tools for the modern cell biologist and immunologist. They can provide a wide range of information which help researchers discern various cellular physical and chemical characteristics. Being an extremely versatile instrument, there are many applications for flow cytometry including cell counting, sorting, viability, apoptosis detection, membrane potential and even binding assays. […]
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Preparing for Publication: Identification and Characterization
In our previous blog post, we explored some of the most commonly used structures for expression and purification of your protein binding partners. The expression systems we addressed included bacterial or eukaryotic cell expression vehicles, which come with distinct pros and cons, depending on the properties or intended future usage of your protein(s). Moreover, we […]
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Preparing for Characterization: Expression and Purification
When proposing a research project, there’s a number of project steps that require advanced planning. Asking questions like, “do I need to express and purify any protein(s)?”, and accounting for this in your project proposal saves you time and unexpected capital expenditures. In this article, we’ll compare the pros and cons of vital project parameters […]
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Comparison Study of Label-Free Biosensors Using Commercially Relevant Binding Systems
Summary Binding kinetics of biotinylated CD16a and IgG were characterized by comparing OpenSPR™ with a standard SPR instrument and a commonly-used BLI instrument. KD values measured using the OpenSPR™, the standard SPR instrument and the commonly-used BLI instrument were found to be 74.7 nM, 48.7 nM and 226 nM, respectively. This study supports the equivalency […]
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Our Mission: Improving Human Life by Helping Scientists Succeed
How is your research changing the world? Over the past 5 years, we’ve had the opportunity to help over 500 researchers worldwide succeed in their area of study. As we worked alongside these innovative researchers, we asked them what they are passionate about and what they need to be successful. We got a lot of […]