Application Notes

Poster: A Novel icIEF and Digital SPR Workflow for Correlating the Charge Structure to the Function of a Bispecific Antibody

Overview Biomolecular charge variant characterization has been a challenge that involves a cumbersome and time-consuming process. In this study, we demonstrate the application of the MauriceFlex™ an...

The dynamic workflow of Alto™ digital SPR for Influenza vaccine development

Overview Rapid changes in influenza antiviral target proteins due to antigenic drift result in the cloaking of the influenza virus from the immune system of vaccinated hosts. Hence, annual formulation...

Quantifying monoclonal antibodies in serum with Alto™ Digital SPR

Overview As the first digital microfluidic (DMF) powered surface plasmon resonance (SPR) instrument in the market, Alto provides users with a quantitation assay to quantify proteins of interest in var...

Poster: Identification of Anti-Idiotype Antibodies using Digital SPR

Overview Anti-idiotypic antibodies (anti-IDs) recognize the unique variable region of another antibody called the idiotype (defined below). Anti-IDs are commonly used in bioanalytical assays to monito...

Rapid, automated capture screening of influenza antiviral targets using Nicoya Alto® digital surface plasmon resonance

Overview Rapid screening and kinetic characterization of medium sized libraries is commonly seen as one of the initial steps within the drug discovery process. Screening assays are used to quickly tes...

Regeneration of His-tagged Streptavidin on OpenSPR® NTA Sensors Sensors

Overview Capture coupling techniques are advantageous to investigate biomolecular interactions as they provide specificity for immobilization on the OpenSPR real-time, label-free biosensor platform. S...

Characterization of HuCAL Fab-MH binding kinetics on Alto™ digital surface plasmon resonance using a Twin-Strep-tag® capture system

Overview In this application note, Alto™ digital SPR was used to characterize HuCAL Fab-MH kinetics using the TwinStrep-tag® Capture Kit, demonstrating the compatibility of the Strep-TactinXT® as ...

Accelerating influenza antibody therapeutics with Alto™ digital microfluidic-powered epitope binning and kinetics analysis

Overview Annual formulation updates for influenza vaccines and related antibody therapies are required to preserve immune recognition against different influenza subtypes. Characterizing the binding k...

Label-free characterization of transmembrane proteins using Alto™ digital SPR

Overview Many human diseases are known to be associated with transmembrane proteins (TPs), making them ideal candidates for drug development. Multi-pass transmembrane proteins are not stable outside o...

Characterization of Alto™ Sample Preparation Precision and Accuracy Enabled by DMF

Overview Precise and accurate liquid handling is crucial to obtaining repeatable, high-quality data with any analytical instrument. This is especially true when preparing samples for surface plasmon r...