Application Notes

Temperature dependence of antibody-antigen binding kinetics on Alto Digital SPR

Overview Biomolecular interaction assays are often conducted at subphysiological temperatures due to equipment limitations, however, temperature significantly influences biomolecular behavior, structu...

Kinetic analysis of a recombinant antibody in undiluted serum on Alto Digital SPR

Overview Characterizing the binding and kinetics of antibody antigen interactions in serum is critical for advancing the development of vaccines, diagnostics, and other biotherapeutics. Screening thes...

Analysis of antibody-antigen binding kinetics on Alto™ Digital SPR using a Human/rabbit IgG VHH capture reagent

Overview Nicoya’s Human/rabbit IgG VHH Capture Kit uses the Nano-CaptureLigand® human IgG/rabbit IgG, Fc-specific VHH (nanobody) from Proteintech to capture human and rabbit antibodies or hu/rb-Fc ...

Designing label-free biosensor assays for Fc receptor binding analysis: Meeting research challenges

Overview Understanding the binding and kinetics of Fc gamma receptors (FcγR) to monoclonal antibodies is critical for advancing the development of targeted therapies aimed at modulating immune respon...

Poster: Rapid Hybridoma Screening with Automated Sample Preparation and Digital SPR Analysis

Overview Alto™ is the worldʼs only Digital SPR™ instrument that uses digital microfluidics (DMF) to handle samples. This allows all SPR sensors, samples, buffers and reagents needed for an experi...

A Novel icIEF Fractionation and SPR-Based Workflow for Correlating the Charge Structure to the Function of a Bispecific Antibody

Overview This application note presents a novel and efficient method for determining binding kinetics and affinity based on the charge heterogeneity of biological molecules. The study examined a thera...

Analysis of HIS-tagged ligands on Alto digital SPR using an anti-HIS capture sensor

Overview Nicoya’s Anti-HIS Capture Kit gives users the ability to capture histidine-tagged (HIS-tagged) ligands directionally, offering an optimal orientation for analyte binding. This method also e...

Analysis of biotin-tagged proteins on Alto digital SPR using a streptavidin functionalized sensor

Overview Nicoya’s Streptavidin Kit allows users to irreversibly capture biotinylated ligands directionally, offering anoptimal orientation for analyte binding. This method also enables users to capt...

Analysis of antibody-antigen binding kinetics on Alto digital SPR using a Protein A capture reagent

Overview Nicoya’s Protein A Capture Kit allows users to capture antibodies or Fc-tagged proteins directionally, offering an optimal orientation for analyte binding. This method also enables users to...

Poster: A Novel icIEF and Digital SPR Workflow for Correlating the Charge Structure to the Function of a Bispecific Antibody

Overview Biomolecular charge variant characterization has been a challenge that involves a cumbersome and time-consuming process. In this study, we demonstrate the application of the MauriceFlex™ an...