Application Note
Binding Kinetics of the GLP-1R Agonist Semaglutide: Practical Methods for Studying Peptide Therapeutics with SPR
Glucagon-like peptide-1 receptor (GLP-1R) agonists are a rapidly expanding class of therapeutics for the treatment of various metabolic diseases. Characterization of their binding kinetics is…
Measuring Aggregation Propensity of a Protein using SUPR-CM
Overview
Comparing pH and Buffer Solutions for Stabilising a Monoclonal Antibody using the SUPR-CM High-Performance Plate Reader
Overview Currently, thermal approaches like differential scanning fluorimetry (DSF) and differential scanning calorimetry (DSC) are often preferred despite drawbacks like non-equilibrium measurements and thermal alteration…
Quantification of Monoclonal Antibody Stability Change After Forced Degradation Studies
Overview Forced degradation studies are routinely employed during the development of new biologics. Along with providing evidence for the mechanisms by which a mAb can…
Multi-method Benchtop Analyses To Gain Novel Insight Into Structure And Internal Stability Of Lipid Nanoparticles For mRNA Delivery
Overview This application note demonstrates how circular dichroism spectroscopy and differential scanning calorimetry can be used together to investigate the structure and thermal stability of…
Rational Optimization of Solvent Conditions for G-Quadruplex Oligonucleotides
Overview As the regulatory role of G-quadruplex structures in crucial biological processes emerges, CD spectroscopy can be expected to gain increased importance in their characterization…
Secondary Structure and Stability of Imperfect G-Quadruplex Oligonucleotides
Overview G-quadruplexes (GQs) are important biomarkers and drug targets because of their regulatory role in transcription. Adherence to the GQ consensus sequence is often used…
Far-UV CD Analysis During Biotherapeutic Development: A Method Capability Study
Overview In this study carried out by Novartis, far- UV CD spectroscopy was qualified for the early development of a monoclonal antibody therapeutic. The high…
Determination of Species-Specific Rate Constants of the Reaction Between Ferrate(VI) and Sulfonamide Antibiotics
Overview Applied Photophysics SX20 stopped-flow spectrometer can be used to study the species-specific rate constants of the reactions between FeVI and SAs. Values of k…
Shining Light on Non-Chemical Steps in Protein Catalysis
Overview Stopped-flow experiments using Applied Photophysics SX20 and Chirascan V100 SF.3 systems allow monitoring of different spectral properties including fluorescence, absorbance, and even far-and near-UV…
High Sensitivity Direct Mount Photodiode Array for the SX20
Overview The directly mounted photodiode array accessory ensures highly efficient light collection for stopped-flow absorbance measurements. The improved design ensures that ultimate sensitivity is achieved…
Stopped-Flow Fluorescence (Förster) Resonance Energy Transfer (FRET)
Overview Stopped-flow FRET is a useful technique for studying many types of biomolecular interactions. The method provides kinetic information regarding distance changes occurring on the…
Identification of AAV Serotypes and Characterisation of their Stability by DSF for use in a Gene Therapy Program
Overview In this application note we used the SUPR-DSF to measure the thermal stability of three AAV serotypes: AAV2, AAV5, and AAV6. The SUPR-DSF measured…
Differential Scanning Fluorimetry Reaches New Peaks on the NISTmAb
Overview The NISTmAb is a widely characterised monoclonal antibody intended to be used as a reference molecule in the development of novel technology for therapeutic…
Using High Throughput Differential Scanning Fluorimetry to Obtain Binding Parameters
Overview Interaction analysis by the SUPR-DSF offers high-speed and label-free measurements across the analyte binding concentration range. The measurements are free in solution and are…
SUPR-DSF Full Spectrum Recording Facilitates Easy Thermal Melt Analysis of Proteins Lacking Tryptophan Residues
Overview This technical note investigates how the full spectrum capabilities of the SUPR-DSF make it easy for researchers to measure unfolding curves for proteins without…
Targeting Optimal Buffers for Downstream Crystallisation Screening
Overview One method of measuring protein stability in a buffer screen is with differential scanning fluorimetry (DSF). Comparison of thermal melting profiles of a protein…
Statistical Repeatability of Tm Values Highlights Utility of Protein Stability Screening Automation
Overview To illustrate the accuracy, and repeatability of microplate-based screening with the SUPR-DSF, 16 x 384-well microplates were prepared with lysozyme using a modular approach…
