Application Notes

Analysis of antibody-antigen binding kinetics on Alto™ Digital SPR using a Human/rabbit IgG VHH capture reagent

Overview Nicoya’s Human/rabbit IgG VHH Capture Kit uses the Nano-CaptureLigand® human IgG/rabbit IgG, Fc-specific VHH (nanobody) from Proteintech to capture human and rabbit antibodies or hu/rb-Fc ...

Designing label-free biosensor assays for Fc receptor binding analysis: Meeting research challenges

Overview Understanding the binding and kinetics of Fc gamma receptors (FcγR) to monoclonal antibodies is critical for advancing the development of targeted therapies aimed at modulating immune respon...

Poster: Rapid Hybridoma Screening with Automated Sample Preparation and Digital SPR Analysis

Overview Alto™ is the worldʼs only Digital SPR™ instrument that uses digital microfluidics (DMF) to handle samples. This allows all SPR sensors, samples, buffers and reagents needed for an experi...

A Novel icIEF Fractionation and SPR-Based Workflow for Correlating the Charge Structure to the Function of a Bispecific Antibody

Overview This application note presents a novel and efficient method for determining binding kinetics and affinity based on the charge heterogeneity of biological molecules. The study examined a thera...

Analysis of HIS-tagged ligands on Alto digital SPR using an anti-HIS capture sensor

Overview Nicoya’s Anti-HIS Capture Kit gives users the ability to capture histidine-tagged (HIS-tagged) ligands directionally, offering an optimal orientation for analyte binding. This method also e...

Analysis of biotin-tagged proteins on Alto digital SPR using a streptavidin functionalized sensor

Overview Nicoya’s Streptavidin Kit allows users to irreversibly capture biotinylated ligands directionally, offering anoptimal orientation for analyte binding. This method also enables users to capt...

Analysis of antibody-antigen binding kinetics on Alto digital SPR using a Protein A capture reagent

Overview Nicoya’s Protein A Capture Kit allows users to capture antibodies or Fc-tagged proteins directionally, offering an optimal orientation for analyte binding. This method also enables users to...

Poster: A Novel icIEF and Digital SPR Workflow for Correlating the Charge Structure to the Function of a Bispecific Antibody

Overview Biomolecular charge variant characterization has been a challenge that involves a cumbersome and time-consuming process. In this study, we demonstrate the application of the MauriceFlex™ an...

The dynamic workflow of Alto™ digital SPR for Influenza vaccine development

Overview Rapid changes in influenza antiviral target proteins due to antigenic drift result in the cloaking of the influenza virus from the immune system of vaccinated hosts. Hence, annual formulation...

Quantifying monoclonal antibodies in serum with Alto™ Digital SPR

Overview As the first digital microfluidic (DMF) powered surface plasmon resonance (SPR) instrument in the market, Alto provides users with a quantitation assay to quantify proteins of interest in var...