Resources
Far-UV CD Analysis During Biotherapeutic Development: A Method Capability Study
Overview In this study carried out by Novartis, far- UV CD spectroscopy was qualified for the early development of a monoclonal antibody therapeutic. The high…
Determination of Species-Specific Rate Constants of the Reaction Between Ferrate(VI) and Sulfonamide Antibiotics
Overview Applied Photophysics SX20 stopped-flow spectrometer can be used to study the species-specific rate constants of the reactions between FeVI and SAs. Values of k…
Shining Light on Non-Chemical Steps in Protein Catalysis
Overview Stopped-flow experiments using Applied Photophysics SX20 and Chirascan V100 SF.3 systems allow monitoring of different spectral properties including fluorescence, absorbance, and even far-and near-UV…
High Sensitivity Direct Mount Photodiode Array for the SX20
Overview The directly mounted photodiode array accessory ensures highly efficient light collection for stopped-flow absorbance measurements. The improved design ensures that ultimate sensitivity is achieved…
Stopped-Flow Fluorescence (Förster) Resonance Energy Transfer (FRET)
Overview Stopped-flow FRET is a useful technique for studying many types of biomolecular interactions. The method provides kinetic information regarding distance changes occurring on the…
Identification of AAV Serotypes and Characterisation of their Stability by DSF for use in a Gene Therapy Program
Overview In this application note we used the SUPR-DSF to measure the thermal stability of three AAV serotypes: AAV2, AAV5, and AAV6. The SUPR-DSF measured…
Differential Scanning Fluorimetry Reaches New Peaks on the NISTmAb
Overview The NISTmAb is a widely characterised monoclonal antibody intended to be used as a reference molecule in the development of novel technology for therapeutic…
Using High Throughput Differential Scanning Fluorimetry to Obtain Binding Parameters
Overview Interaction analysis by the SUPR-DSF offers high-speed and label-free measurements across the analyte binding concentration range. The measurements are free in solution and are…
SUPR-DSF Full Spectrum Recording Facilitates Easy Thermal Melt Analysis of Proteins Lacking Tryptophan Residues
Overview This technical note investigates how the full spectrum capabilities of the SUPR-DSF make it easy for researchers to measure unfolding curves for proteins without…
Targeting Optimal Buffers for Downstream Crystallisation Screening
Overview One method of measuring protein stability in a buffer screen is with differential scanning fluorimetry (DSF). Comparison of thermal melting profiles of a protein…
Statistical Repeatability of Tm Values Highlights Utility of Protein Stability Screening Automation
Overview To illustrate the accuracy, and repeatability of microplate-based screening with the SUPR-DSF, 16 x 384-well microplates were prepared with lysozyme using a modular approach…
Formulation screen of Trastuzumab using the SUPR-DSF
Overview In this report we show the analysis of a thermal denaturation-based formulation screen of then commercially available therapeutic antibody Trastuzumab, in 96 different conditions…
Finding Formulations Faster with SUPR-DSF
Overview In this application note we show the analysis of a thermal denaturation-based formulation screen of the commercially available therapeutic antibody Infliximab with the FORMOscreen®…
Speeding Up Early Stage Biotherapeutic Discovery with Next Generation Differential Scanning Fluorimetry
Overview Existing technologies for measuring stability of biologics have all proved useful in identifying candidates that have higher stability than others under certain parameters but…
Applied Photophysics Brochure
Explore the Applied Photophysics portfolio, including Chirascan CD, SUPR-DSF protein stability, and SX stopped-flow kinetics.
Alto Brochure
Meet the world’s first digital SPR system offering a new approach: cartridge-based kinetics and affinity data from only 2µL of sample.
Nicoya Alto CBX Cartridge Sensor Performance
With Alto, Nicoya’s Digital surface plasmon resonance™ (SPR) platform, the entirety of the fluidic control and sensing is done on single-use disposable cartridges. High accuracy…
Uncovering New Tools to Analyze Cytokine–Receptor Interactions: How Conigen Bioscience Used Nicoya’s Alto™ Digital SPR to Characterize Novel Dimeric Receptors
Presenters: Michael Piazza, PhD AVP, Applications Development Nicoya Lifesciences Alyssa Ball, PhD Professor, Assay Development Scientist Conigen Bioscience Overview Understanding cytokine-receptor interactions is critical for…
