Resources
SUPR-DSF Full Spectrum Recording Facilitates Easy Thermal Melt Analysis of Proteins Lacking Tryptophan Residues
Overview This technical note investigates how the full spectrum capabilities of the SUPR-DSF make it easy for researchers to measure unfolding curves for proteins without…
Targeting Optimal Buffers for Downstream Crystallisation Screening
Overview One method of measuring protein stability in a buffer screen is with differential scanning fluorimetry (DSF). Comparison of thermal melting profiles of a protein…
Statistical Repeatability of Tm Values Highlights Utility of Protein Stability Screening Automation
Overview To illustrate the accuracy, and repeatability of microplate-based screening with the SUPR-DSF, 16 x 384-well microplates were prepared with lysozyme using a modular approach…
Formulation screen of Trastuzumab using the SUPR-DSF
Overview In this report we show the analysis of a thermal denaturation-based formulation screen of then commercially available therapeutic antibody Trastuzumab, in 96 different conditions…
Finding Formulations Faster with SUPR-DSF
Overview In this application note we show the analysis of a thermal denaturation-based formulation screen of the commercially available therapeutic antibody Infliximab with the FORMOscreen®…
Speeding Up Early Stage Biotherapeutic Discovery with Next Generation Differential Scanning Fluorimetry
Overview Existing technologies for measuring stability of biologics have all proved useful in identifying candidates that have higher stability than others under certain parameters but…
Applied Photophysics Brochure
Explore the Applied Photophysics portfolio, including Chirascan CD, SUPR-DSF protein stability, and SX stopped-flow kinetics.
Alto Brochure
Text about the Alto Brochure
Nicoya Alto CBX Cartridge Sensor Performance
With Alto, Nicoya’s Digital surface plasmon resonance™ (SPR) platform, the entirety of the fluidic control and sensing is done on single-use disposable cartridges. High accuracy…
Uncovering New Tools to Analyze Cytokine–Receptor Interactions: How Conigen Bioscience Used Nicoya’s Alto™ Digital SPR to Characterize Novel Dimeric Receptors
Presenters: Michael Piazza, PhD AVP, Applications Development Nicoya Lifesciences Alyssa Ball, PhD Professor, Assay Development Scientist Conigen Bioscience Overview Understanding cytokine-receptor interactions is critical for…
Analysis of antibody-antigen binding kinetics on Alto Digital SPR using mouse Fc-specific capture reagents
Overview Mouse Fc-specific capture reagents can be used to capture mouse antibodies or Fc-tagged proteins directionally. These capture reagents offer an optimal orientation for analyte…
Analysis of antibody-antigen binding kinetics on Alto Digital SPR using mouse Fc-specific capture reagents
Overview Mouse Fc-specific capture reagents can be used to capture mouse antibodies or Fc-tagged proteins directionally. These capture reagents offer an optimal orientation for analyte…
Temperature dependence of antibody-antigen binding kinetics on Alto Digital SPR
Overview Biomolecular interaction assays are often conducted at subphysiological temperatures due to equipment limitations, however, temperature significantly influences biomolecular behavior, structure, function, and interactions. For…
Kinetic analysis of a recombinant antibody in undiluted serum on Alto Digital SPR
Overview Characterizing the binding and kinetics of antibody antigen interactions in serum is critical for advancing the development of vaccines, diagnostics, and other biotherapeutics. Screening…
Analysis of antibody-antigen binding kinetics on Alto™ Digital SPR using a Human/rabbit IgG VHH capture reagent
Overview Nicoya’s Human/rabbit IgG VHH Capture Kit uses the Nano-CaptureLigand® human IgG/rabbit IgG, Fc-specific VHH (nanobody) from Proteintech to capture human and rabbit antibodies or…
Designing label-free biosensor assays for Fc receptor binding analysis: Meeting research challenges
Overview Understanding the binding and kinetics of Fc gamma receptors (FcγR) to monoclonal antibodies is critical for advancing the development of targeted therapies aimed at…
Poster: Rapid Hybridoma Screening with Automated Sample Preparation and Digital SPR Analysis
Overview Alto™ is the worldʼs only Digital SPR™ instrument that uses digital microfluidics (DMF) to handle samples. This allows all SPR sensors, samples, buffers and…
A Novel icIEF Fractionation and SPR-Based Workflow for Correlating the Charge Structure to the Function of a Bispecific Antibody
Overview This application note presents a novel and efficient method for determining binding kinetics and affinity based on the charge heterogeneity of biological molecules. The…
