Tech Notes

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Analysis of antibody-antigen binding kinetics on Alto Digital SPR using a Human/rabbit IgG VHH capture reagent

Nicoya’s Protein A Capture Kit allows users to capture antibodies or Fc-tagged proteins directionally, offering an optimal orientation for analyte binding. This method also enables…

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Analysis of antibody-antigen binding kinetics on Alto Digital SPR using a Protein A capture reagent

Nicoya’s Protein A Capture Kit allows users to capture antibodies or Fc-tagged proteins directionally, offering an optimal orientation for analyte binding. This method also enables…

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Analysis of biotin-tagged proteins on Alto digital SPR using a streptavidin functionalized sensor

Nicoya’s Streptavidin Kit allows users to irreversibly capture biotinylated ligands directionally, offering anoptimal orientation for analyte binding. This method also enables users to capture ligands…

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Analysis of HIS-tagged ligands on Alto digital SPR using an anti-HIS capture sensor

Nicoya’s Anti-HIS Capture Kit gives users the ability to capture histidine-tagged ligands directionally, offering an optimal orientation for analyte binding. This method also enables users…

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Characterization and Quantitation of AAVs on Alto Digital SPR using AAVX & AAV9 capture surfaces

Nicoya’s AAVX & AAV9 Capture Kits use AAV-specific VHH molecules to capture AAVs on the sensor surface. This capture strategy offers an optimal orientation for…

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Quantifying monoclonal antibodies in serum with Alto™ Digital SPR

Overview As the first digital microfluidic (DMF) powered surface plasmon resonance (SPR) instrument in the market, Alto provides users with a quantitation assay to quantify…

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Analysis of proteins containing Twin-Strep-Tags on Alto Digital SPR using capture immobilization strategies

Overview The Twin-Strep-Tag® technology from IBA Lifesciences utilizes Strep-Tactin®XT to capture proteins labelled with a Twin-Strep-Tag directionally. Proteintech, a leading provider of antibody reagents, has…

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Characterization of Alto™ Sample Preparation Precision and Accuracy Enabled by DMF

Overview Precise and accurate liquid handling is crucial to obtaining repeatable, high-quality data with any analytical instrument. This is especially true when preparing samples for…