Overview
One method of measuring protein stability in a buffer screen is with differential scanning fluorimetry (DSF). Comparison of thermal melting profiles of a protein in different buffers can indicate the conditions that increase protein stability. We show how the SUPR-DSF can be used to rapidly screen bovine
Beta-lactoglobulin (BLG) in a variety of buffer conditions to increase stability and subsequently aid in improving homogeneity in solution (Figure 1). The system’s 384-well plate format allows simple screening of commercially available buffer screens, plus replicates, in one thermal ramp experiment. These screens have minimal set-up time and require no extrinsic dyes. A preliminary buffer screen can be used to ensure proteins are stored in optimal buffer conditions prior to any structural methodologies being implemented.
