Overview
In this application note we used the SUPR-DSF to measure the thermal stability of three AAV serotypes: AAV2, AAV5, and AAV6. The SUPR-DSF measured the intrinsic fluorescence of each serotype during a thermal ramp to generate melt curves. In addition, we performed a concentration dilution series on AAV2 and AAV6 to determine the minimal sample requirements. All samples were performed at a well volume of 10µL in triplicate on a single 384-well microplate emphasising the high-throughput possibilities of the instrument.
