Don’t just keep up, stay ahead with SX20
Unlock biology in real time. Capture millisecond reactions with unmatched precision.
Discovery done right
The world’s most-cited stopped-flow system, SX20 gives scientists insight into the fastest molecular events, revealing reaction pathways, intermediates, and mechanisms that shape biological function. With high sensitivity, dual-detection capabilities, and reproducible measurements, it provides reliable data on protein interactions, folding, ligand binding, enzyme kinetics, and chemical reactions—empowering better decision-making in research and formulation development.
Gain rich, multi-dimensional data
Simultaneously monitor multiple signals—such as fluorescence, absorbance, or FRET—to capture complementary insights in a single experiment. Dual-detection maximizes efficiency and gives you deeper mechanistic understanding faster.
High-speed spectral acquisition reveals reaction intermediates in real time. SX20 captures the full evolution of complex mechanisms across the UV–Vis range, enabling global analysis and multi-step kinetic modeling.
Simplify complex experiments
Automatic dead-time and drive-volume measurements, combined with the integrated pneumatic mixer, reduce setup complexity and eliminate manual calibration—so you can focus on analyzing results rather than troubleshooting the instrument.
Flexibility for every experiment
Choose from a wide range of light sources, detectors, and accessories, supported by Pro-K software. SX20 adapts to diverse biological and chemical systems, enabling thermal, pH, or chemical stability studies and custom workflows with ease. Download the SX20 Consumables & Accessories Brochure to learn more.
Light sources designed for your workflow
SX20’s interchangeable light sources give researchers the freedom to optimize each experiment. As shown below, the xenon lamp provides substantially higher intensity and signal-to-noise performance for fluorescence polarization kinetics, revealing subtle structural changes with greater precision. LED illumination remains a robust, cost-effective option for routine assays or strongly fluorescent samples. With SX20, you can tailor the setup to the demands of your biology without compromising data quality.
SX20 options
Xenon
Versatile, Broad-Spectrum Performance
Stable, intense emission across a broad wavelength range makes the xenon source ideal for both absorbance and fluorescence applications. It delivers high sensitivity, broad scanning capability, and robust performance, with a wide temperature range and a five-year warranty.
LED
Targeted Illumination for Enhanced Fluorescence
Monochromatic LED sources provide high intensity and stability at selected wavelengths, maximizing fluorescence detection. Compact, cost-effective, and fast-starting, the SX20 LED offers low inner-filtering, low dead-time, and comprehensive ProData software support.
Explore key molecular interactions
SX20 lets you study fast biochemical reactions in real time, providing actionable insights into enzymes, protein folding, and protein-ligand interaction.
Enzyme Kinetics
Uncover Enzyme Reaction Details
Measure rapid enzymatic steps in real time, revealing intermediate states, rate-limiting steps, and binding affinities. SX20 enables precise kinetic analysis for drug discovery, synthetic biology, and complex biochemical studies.
Protein Folding
Track Folding Pathways Precisely
Follow protein folding and unfolding in real time to identify intermediate states and misfolding events. SX20 helps researchers understand folding mechanisms, energy landscapes, and potential drug targets for misfolding diseases.
Protein-Ligand interactions
Quantify Binding Dynamics Accurately
Measure association and dissociation rates, detect subtle spectroscopic changes, and perform FRET experiments. SX20 delivers high-resolution insights into protein-ligand mechanisms, enabling informed decisions in drug design and molecular research.
Precision kinetics, trusted worldwide
SX20 combines rapid stopped-flow mixing, dual detection, and optimized optics to deliver reproducible, high-sensitivity kinetic measurements. Trusted by over 1,000 labs worldwide, it powers 12,000+ peer-reviewed discoveries and is the most-cited stopped-flow system in the world.
12,000+
Peer-reviewed Publications
As Seen In:
Resources
SX20 Brochure
Learn more about SX20
Shining Light on Non-Chemical Steps in Protein Catalysis
Overview Stopped-flow experiments using Applied Photophysics SX20 and Chirascan V100 SF.3 systems allow monitoring of different spectral properties including fluorescence, absorbance, and even far-and near-UV…
Stopped-Flow Fluorescence (Förster) Resonance Energy Transfer (FRET)
Overview Stopped-flow FRET is a useful technique for studying many types of biomolecular interactions. The method provides kinetic information regarding distance changes occurring on the…
Capture fast reactions. Reveal molecular insights.
Accelerate your kinetic studies with precision, sensitivity, and confidence.
