Resources
Analysis of antibody-antigen binding kinetics on Alto™ Digital SPR using a Human/rabbit IgG VHH capture reagent
Overview Nicoya’s Human/rabbit IgG VHH Capture Kit uses the Nano-CaptureLigand® human IgG/rabbit IgG, Fc-specific VHH (nanobody) from Proteintech to capture human and rabbit antibodies or…
Designing label-free biosensor assays for Fc receptor binding analysis: Meeting research challenges
Overview Understanding the binding and kinetics of Fc gamma receptors (FcγR) to monoclonal antibodies is critical for advancing the development of targeted therapies aimed at…
Poster: Rapid Hybridoma Screening with Automated Sample Preparation and Digital SPR Analysis
Overview Alto™ is the worldʼs only Digital SPR™ instrument that uses digital microfluidics (DMF) to handle samples. This allows all SPR sensors, samples, buffers and…
A Novel icIEF Fractionation and SPR-Based Workflow for Correlating the Charge Structure to the Function of a Bispecific Antibody
Overview This application note presents a novel and efficient method for determining binding kinetics and affinity based on the charge heterogeneity of biological molecules. The…
Analysis of HIS-tagged ligands on Alto digital SPR using an anti-HIS capture sensor
Overview Nicoya’s Anti-HIS Capture Kit gives users the ability to capture histidine-tagged (HIS-tagged) ligands directionally, offering an optimal orientation for analyte binding. This method also…
Analysis of biotin-tagged proteins on Alto digital SPR using a streptavidin functionalized sensor
Overview Nicoya’s Streptavidin Kit allows users to irreversibly capture biotinylated ligands directionally, offering anoptimal orientation for analyte binding. This method also enables users to capture…
Analysis of antibody-antigen binding kinetics on Alto digital SPR using a Protein A capture reagent
Overview Nicoya’s Protein A Capture Kit allows users to capture antibodies or Fc-tagged proteins directionally, offering an optimal orientation for analyte binding. This method also…
Poster: A Novel icIEF and Digital SPR Workflow for Correlating the Charge Structure to the Function of a Bispecific Antibody
Overview Biomolecular charge variant characterization has been a challenge that involves a cumbersome and time-consuming process. In this study, we demonstrate the application of the…
The dynamic workflow of Alto™ digital SPR for Influenza vaccine development
Overview Rapid changes in influenza antiviral target proteins due to antigenic drift result in the cloaking of the influenza virus from the immune system of…
Quantifying monoclonal antibodies in serum with Alto™ Digital SPR
Overview As the first digital microfluidic (DMF) powered surface plasmon resonance (SPR) instrument in the market, Alto provides users with a quantitation assay to quantify…
Poster: Identification of Anti-Idiotype Antibodies using Digital SPR
Overview Anti-idiotypic antibodies (anti-IDs) recognize the unique variable region of another antibody called the idiotype (defined below). Anti-IDs are commonly used in bioanalytical assays to…
Rapid, automated capture screening of influenza antiviral targets using Nicoya Alto® digital surface plasmon resonance
Overview Rapid screening and kinetic characterization of medium sized libraries is commonly seen as one of the initial steps within the drug discovery process. Screening…
Regeneration of His-tagged Streptavidin on OpenSPR® NTA Sensors Sensors
Overview Capture coupling techniques are advantageous to investigate biomolecular interactions as they provide specificity for immobilization on the OpenSPR real-time, label-free biosensor platform. Streptavidin sensors…
Analysis of proteins containing Twin-Strep-Tags on Alto Digital SPR using capture immobilization strategies
Overview The Twin-Strep-Tag® technology from IBA Lifesciences utilizes Strep-Tactin®XT to capture proteins labelled with a Twin-Strep-Tag directionally. Proteintech, a leading provider of antibody reagents, has…
Accelerating influenza antibody therapeutics with Alto™ digital microfluidic-powered epitope binning and kinetics analysis
Overview Annual formulation updates for influenza vaccines and related antibody therapies are required to preserve immune recognition against different influenza subtypes. Characterizing the binding kinetics…
Label-free characterization of transmembrane proteins using Alto™ digital SPR
Overview Many human diseases are known to be associated with transmembrane proteins (TPs), making them ideal candidates for drug development. Multi-pass transmembrane proteins are not…
Characterization of Alto™ Sample Preparation Precision and Accuracy Enabled by DMF
Overview Precise and accurate liquid handling is crucial to obtaining repeatable, high-quality data with any analytical instrument. This is especially true when preparing samples for…
Epitope Characterization of Influenza Antiviral Targets Using Alto™
Overview Rapid changes in influenza antiviral target proteins due to antigenic drift result in cloaking of the influenza virus from the immune system of vaccinated…
