Resources
Analysis of Antibody-Antigen Binding Kinetics on Digital SPR using the Protein G Capture Kit
Nicoya’s Protein G Capture Kit allows users to capture antibodies or Fc-tagged proteins directionally, offering an optimal orientation for analyte binding. This method also enables…
Thermal Stability Analysis of Tryptophan-Free Proteins Using SUPR-DSF
Intrinsic differential scanning fluorimetry (intrinsic DSF) is most commonly associated with proteins containing tryptophan residues, whose strong fluorescence signal provides excellent sensitivity for monitoring thermal…
Relatively Speaking: Secondary Structure Decomposition and HOS Comparison Analysis for CD Comparability Studies
Circular dichroism (CD) spectroscopy is a well-established technique for the structural characterization of chiral molecules and has become an indispensable part in the biophysical toolbox…
Integrated Structural and Functional Characterization of a GLP-1 Analogue Using LC/MS and SPR
This application note presents an integrated analytical workflow combining an Agilent 1290 Infinity II bio LC system, an Agilent 6545XT AdvanceBio LC/Q-TOF, and a Nicoya…
Alto Brochure
Alto is Nicoya’s fully automated digital SPR platform for high-confidence kinetic and affinity analysis at scale.
OpenSPR Brochure
OpenSPR brings reliable, label-free SPR measurements to a compact benchtop format, enabling first-pass interaction validation and structure–function exploration.
Digital SPR STV Cartridge Sensor Performance
The reproducibility of several sensor metrics was assessed across four streptavidin (STV) cartridges from different lots. From this study, sensitivity, immobilization, and analyte response were…
Integrated Biophysical Characterization of Tocilizumab and a Biosimilar Candidate: A Multi-Dimensional Study of Binding Kinetics, Thermal Stability, and Higher Order Structure
The development of biosimilar monoclonal antibodies requires rigorous demonstration of structural and functional equivalence to the reference product under a regulatory “totality of evidence” framework.…
SUPR-DSF Flyer
Plate-based intrinsic DSF delivers both melting temperature (Tm) and non-reversibility onset (Tnr)—providing faster, more affordable stability analysis directly in your standard microplate workflow.
Comprehensive Assessment of Protein Conformational Stability and Unfolding Reversibility in Biotherapeutic Development via Intrinsic DSF and MSF
The development of biotherapeutics requires rigorous evaluation of both conformational and colloidal stability. In this application note, we introduce a new temperature cycling capability for…
Revo Flyer
Meet Revo – SPR reimagined for real-world biologics.
FastHDX Flyer
Capture every transient state in protein dynamics with FastHDX.
Analysis of antibody-antigen binding kinetics on Alto Digital SPR using a Human/rabbit IgG VHH capture reagent
Nicoya’s Protein A Capture Kit allows users to capture antibodies or Fc-tagged proteins directionally, offering an optimal orientation for analyte binding. This method also enables…
Nicoya Brochure
Explore the full Nicoya portfolio, including Alto and Revo digital SPR, Chirascan CD, OpenSPR, SUPR-DSF protein stability, FastHDX, and SX stopped-flow kinetics.
Mastering Binding Kinetics Assays
Discover how to efficiently characterize biomolecular interactions with our comprehensive eBook. Learn how the Alto™ digital SPR system simplifies interaction analysis by integrating digital microfluidics…
Analysis of antibody-antigen binding kinetics on Alto Digital SPR using a Protein A capture reagent
Nicoya’s Protein A Capture Kit allows users to capture antibodies or Fc-tagged proteins directionally, offering an optimal orientation for analyte binding. This method also enables…
Epitope Binning
Discover how the Alto™ Digital SPR system can advance your antibody discovery workflows with the Epitope Binning application. Learn how Alto simplifies experiment design and…
Analysis of biotin-tagged proteins on Alto digital SPR using a streptavidin functionalized sensor
Nicoya’s Streptavidin Kit allows users to irreversibly capture biotinylated ligands directionally, offering anoptimal orientation for analyte binding. This method also enables users to capture ligands…
