Advanced protein stability, made simple
Plate-based intrinsic DSF (differential scanning fluorimetry) delivers clearer stability insights faster, more affordably, and directly in your standard microplate workflow.
SUPR-DSF is a benchtop microplate reader that measures protein stability using intrinsic fluorescence – no dyes, no proprietary consumables. From formulation and manufacturing to automation, it delivers reliable melting data with minimal sample prep, helping scientists move faster with confidence and clarity.
Dye-free, in-plate simplicity
SUPR-DSF eliminates extrinsic dyes and extra preparation steps by measuring intrinsic protein fluorescence directly in a single 384-well plate. This reduces hands-on time, lowers error risk, and ensures signals reflect true protein unfolding that is unaffected by buffers, additives, or pH.
Full-spectrum data you can trust
By capturing the complete intrinsic fluorescence spectrum across the full temperature range, SUPR-DSF reveals subtle stability differences others miss. Rich, high-integrity data enables flexible analysis approaches and confident decision-making, even at therapeutic protein concentrations or in challenging formulations.
Scale Stability Screening with Automation
SUPR-DSF fits seamlessly into automated stability screening workflows, enabling labs to scale condition screening and candidate ranking without adding operational complexity. Its plate-based format supports straightforward integration with liquid handlers for repeatable sample setup and consistent execution across large studies. The result is faster iteration, improved reproducibility, and a stability workflow that can grow with your pipeline.
Designed for cost-efficient screening
Run up to 384 samples in a single experiment using standard, low-cost qPCR plates and seals, no proprietary consumables required. With minimal sample volumes and seamless integration into existing lab automation, SUPR-DSF delivers exceptional efficiency without sacrificing performance.
Real-world protein applications
SUPR-DSF powers key stages of protein discovery and development, delivering fast, reliable stability and interaction insights that accelerate innovation and reduce experimental bottlenecks.
Protein Engineering & Construct Screening
Rapid Construct Stability
SUPR-DSF ranks hundreds of early protein constructs directly in-plate, revealing thermal stability and potential self-association in hours, enabling faster, confident decisions during protein engineering and discovery programs.
Formulation & Stability Prediction
Predictive Formulation Insights
Test multiple buffers, excipients, and conditions efficiently in a single 384-well plate. SUPR-DSF delivers fast, accurate stability data while reducing errors, costs, and manual sample handling.
Protein-Ligand Binding Studies
Sensitive Interaction Analysis
Detect weak or strong protein-ligand interactions in solution using thermal shift analysis. SUPR-DSF supports screening and complementary orthogonal binding assessments with minimal sample volumes.
Precision DSF technology
SUPR-DSF combines multiple DSF analysis methods—intensity, ratio, barycentric mean, thermodynamic modeling, and derivative fitting—to generate accurate, reproducible melting curves, empowering scientists with high-confidence protein stability measurements and actionable insights
Resources
Analysis of antibody-antigen binding kinetics on Alto Digital SPR using mouse Fc-specific capture reagents
Overview Mouse Fc-specific capture reagents can be used to capture mouse antibodies or Fc-tagged proteins directionally. These capture reagents offer an optimal orientation for analyte…
Nicoya Alto CBX Cartridge Sensor Performance
With Alto, Nicoya’s Digital surface plasmon resonance™ (SPR) platform, the entirety of the fluidic control and sensing is done on single-use disposable cartridges. High accuracy…
Uncovering New Tools to Analyze Cytokine–Receptor Interactions: How Conigen Bioscience Used Nicoya’s Alto™ Digital SPR to Characterize Novel Dimeric Receptors
Presenters: Michael Piazza, PhD AVP, Applications Development Nicoya Lifesciences Alyssa Ball, PhD Professor, Assay Development Scientist Conigen Bioscience Overview Understanding cytokine-receptor interactions is critical for…
Unlock Protein Stability Insights
Gain clear, actionable data on protein folding and interactions—streamlining experiments, saving time, and powering confident decisions throughout your discovery workflow.
