Overview
Stopped-flow experiments using Applied Photophysics SX20 and Chirascan V100 SF.3 systems allow monitoring of different spectral properties including fluorescence, absorbance, and even far-and near-UV CD.With these tools. This study outlines the transition between fully folded and locally unfolded states of Tsa1, in its sulfenic acid form CP-SOH, is not in rapid equilibrium as previously hypothesised, but is almost irreversible (kFF~0s-1)and in direct competition with the hyperoxidation step.
