Presenter:
Karl Maluf
Director of the Biophysical Characterisation Core
KBI Biopharma
Overview
In this talk, Karl discusses the challenges of analysing AAV heterogeneity and the critical role of Analytical Ultracentrifugation (AUC) in quantifying size distributions, capsid integrity, and genome packaging. Comparing AUC with size exclusion chromatography (SEC), he highlights how multi-wavelength sedimentation analysis enhances resolution and accuracy in identifying AAV subspecies.
Key Insights from Karl’s Talk:
- AUC as the Gold Standard: Multi-wavelength sedimentation velocity analysis improves detection of empty, partially packaged, and fully packaged capsids.
- High-Speed AUC Analysis: Faster spin rates and temperature adjustments enhance peak resolution and reveal over-packaged material.
- Comparing Characterisation Methods: AUC outperforms SEC in distinguishing AAV subspecies without modification.
- New Approaches in Gene Therapy: Advanced density gradient and multi-wavelength methods refine AAV analysis for safer, more effective therapeutics.
Discover how Karl’s insights are shaping the future of AAV characterisation and gene therapy innovation.
