Blog
Educational material, comparison studies and publications on SPR and other related techniques for researchers.
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SPR Expert Interview: Dr. George Espie
Meet SPR Expert: Dr. George Espie. Dr. Espie is a Professor of Biology at the University of Toronto Mississauga & Graduate Program Cell & Systems Biology at the University of Toronto. When he isn’t lecturing, you can find him in his lab working on innovative protein-protein research. With over 23 publications we interviewed Dr. Espie […]
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Waterloo Professor Thorsten Dieckmann Says SPR Data Was Key Link Needed For Publication
Dr. Dieckmann, Mike Piazza and Dr. Guillemette from the University of Waterloo published a paper that uses OpenSPR to study the structure and function of the protein calmodulin (CaM). CaM is a small protein important for many physiological and pathological processes, and serves as a calcium sensor and control element for nitric oxide synthases (NOS). […]
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How OpenSPR helped Dr. Espie Publish his Groundbreaking Research
Do you want to publish your research sooner? Dr. George Espie from the University of Toronto reveals how OpenSPR helped him accelerate his research and publish within 3 months.The research paper “The structure, kinetics and interactions of the β-carboxysomal β-carbonic anhydrase, CcaA” published in Biochemical Journal uses surface plasmon resonance data generated with OpenSPR to study the […]
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Comparison Study of SPR Instruments Using Commercially Relevant Binding Systems
Comparison Study of SPR Instruments Using Commercially Relevant Binding Systems: A protein-protein interaction was analyzed on the OpenSPR™ and the standard SPR insturment. Kinetic analysis was used to determine the on rate, off rate, and affinity constant of the protein-protein interaction. Both data sets generated from OpenSPR™ and the comparison SPR instrument show excellent fits […]
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Binding Kinetics of Protein-Lipid Interactions Using Surface Plasmon Resonance
Surface plasmon resonance (SPR) can be used to analyze all types of interactions ranging from protein-protein, protein-small molecule, protein-nucleic acid, protein-aptamers, protein-lipids, and many more. One area of research is the study of lipid binding kinetics, and many scientists are implementing techniques such as SPR to define the affinity and specificity of these interactions. Why […]
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8 Advantages of Using SPR in Your Own Lab
What do you do if you need a specific piece of equipment, but can’t afford to purchase it outright? You may apply for a research grant, ask fellow researchers to borrow their equipment, or avoid conducting the experiments altogether.
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Binding kinetics of glycoprotein interactions using surface plasmon resonance
Surface plasmon resonance (SPR) can be used to analyze all types of interactions ranging from protein-protein, protein-small molecule, protein-nucleic acid, protein-aptamer, protein-lipid, carbohydrate-protein (carbohydrate-lectin) , carbohydrate-carbohydrate and many more. One area of research is the study of glycoprotein binding kinetics, and many scientists are implementing techniques such as SPR to define the kinetic constants of […]
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SPR Expert Interview: Dr. Thorsten Dieckmann
Dr. Thorsten Dieckmann, University of Waterloo Associate Professor in Chemistry and Biochemistry, has used Nicoya’s OpenSPR™ to recently publish “Structural Consequences of Calmodulin EF Hand Mutations” with M. Piazza, V. Taiakina, and J. G. Guillemette. With over 40 publications, we interviewed Dr. Dieckmann about his RNA-ligand research and experience using OpenSPR. He is now expanding […]
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App Note: Binding Kinetics of Protein-Protein Interactions using OpenSPR™
Are protein binding kinetics important for your research? Benchtop SPR is a user-friendly tool that allows researchers to characterize biomolecular interactions in real-time measurements. Forget CO-IPs that days to run and only give end-point measurements, or techniques that use too much precious sample like ITC. In this blog post, we discuss how OpenSPR™ can be […]
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Nicoya’s Q & A: Researcher Profile – Dr. Guy Guillemette
Dr. Guy Guillemette, University of Waterloo Associate Professor in Chemistry and Biochemistry, has used Nicoya’s OpenSPR™ to publish “Structural Studies of a Complex Between Endothelial Nitric Oxide Synthase and Calmodulin at Physiological Calcium Concentration” with M. Piazza and T. Dieckmann. With over 70 publications, we interviewed Dr. Guillemette about his protein-peptide research and experience using […]