Temperature Dependent Binding Kinetics of Protein-Protein Interactions Using OpenSPR


OpenSPR™ is a powerful instrument providing indepth label-free binding kinetics for a variety of different molecular interactions. One common applications of surface plasmon resonance is to investigate the effect of temperature on the binding kinetics and affinity of an interaction, which can also be used to determine thermodynamic parameters. In this application note, we demonstrate how OpenSPR™ is used to analyze the effect of temperature of the kinetics and affinity of a protein-protein interaction by using the Nicoya Temperature Control Add-On.