Binding Kinetics of Therapeutic Monoclonal Antibodies to the CD16a Fc Receptor using OpenSPR


CD16a (FcyRIIIA) is an Fc receptor protein found on the surface of certain immune cells. CD16a binds to the Fc region of immunoglobulin G (IgG). CD16a-IgG binding initiates antibody-dependent cellular cytotoxicity (ADCC), an important effector mechanism of the immune system.
CD16a assays are essential for the development and production of therapeutic monoclonal antibody (mAbs). In this application note, OpenSPRâ„¢ is used to successfully determine the binding kinetics and affinity of human CD16a interacting with human IgG (Fc region). OpenSPR allows users to easily and accurately measure the binding kinetics of numerous receptor-ligand systems.