Notes

Label-Free Characterization of the Interleukin-6 Receptor and Anti-IL-6R Antibody Drug Tocilizumab using Alto™

Single-cycle kinetics of IL-6 (analyte) binding to immobilized IL-6R (ligand) on Alto
Single-cycle kinetics of IL-6 (analyte) binding to immobilized IL-6R (ligand) on Alto. Analyte was titrated from 1.85 nM to 150 nM. Black curve is the Langmuir 1:1 binding fit model analyzed in the Nicoya Analysis Software.

Overview

Therapeutic monoclonal antibodies are one of the fastest growing classes of drugs, and interleukins and their signal pathways are a preeminent target for treatments due to the vast number of problems their improper regulation present. Surface plasmon resonance (SPR) is a powerful tool that is well-suited for analyzing the binding interactions associated with signaling proteins, their receptors and related effectors.

In this application note, we demonstrate Alto’s applicability to the development of monoclonal antibody therapeutics, with a focus on interleukin and interleukin-related therapies