OpenSPR
Obtain publication-quality binding kinetics & affinity data on your benchtop.
BOOK WEB DEMOConfidence in every data point: uncompromised SPR
Surface plasmon resonance (SPR) is essential in biologics research for binding kinetics, analyte concentration, and epitope mapping. However, high costs and technical complexity often limit access, requiring many labs to rely on shared facilities or outsourced analysis.
The OpenSPR® platform was designed specifically to reduce the
cost and complexity of SPR assays to allow more labs access to the benefits of label-free SPR analysis. Utilizing localized SPR (LSPR), the OpenSPR platform produces equivalent, if not more robust, data compared to leading SPR systems at a fraction of the cost to purchase and maintain.
The fully automated OpenSPR-XT system pairs the base OpenSPR instrument with an autosampler, to allow for 24/7 operation.
Surface plasmon resonance (SPR) is essential in biologics research for binding kinetics, analyte concentration, and epitope mapping. However, high costs and technical complexity often limit access, requiring many labs to rely on shared facilities or outsourced analysis.
The OpenSPR® platform was designed specifically to reduce the cost and complexity of SPR assays to allow more labs access to the benefits of label-free SPR analysis. Utilizing localized SPR (LSPR), the OpenSPR platform produces equivalent, if not more robust, data compared to leading SPR systems at a fraction of the cost to purchase and maintain.
The fully automated OpenSPR-XT system pairs the base OpenSPR instrument with an autosampler, to allow for 24/7 operation.
OpenSPR applications
OpenSPR can reliably detect molecules 1 kDa and above, including antibodies, proteins, virus particles, peptides, oligonucleotides, and carbohydrates. Tolerant of crude media, OpenSPR is compatible with serum, cell lysate, and cell culture supernatant.
A few select applications where OpenSPR excels include:
- Mechanism of signaling, action analyses
- Hybridoma, phage display screening
- Antibody-antigen, antibody-Fc receptor kinetics
- Antibody titer
- Antigen characterization
- Antibody immune response in serum
- Virus particle characterization
- Reagent characterization (quality assessment)
LSPR benchtop design like no other
LSPR operates similarly to traditional SPR, using light to measure biomolecular interactions in real time. The key difference is that LSPR detects absorbance wavelength shifts from metal nanoparticles, while SPR measures angle changes from a gold film. Both methods yield comparable kinetics and affinity data, but their optical properties have distinct implications.
By design, OpenSPR LSPR systems are:
- Smaller and more affordable, with lower costs for instrument, consumables and service
- Less sensitive to environmental variations like temperature and vibration
- More cost-effective to operate with nanotechnology-based sensors
- Easier to use and maintain with less optical hardware complexity inside
LSPR operates similarly to traditional SPR, using light to measure biomolecular interactions in real time. The key difference is that LSPR detects absorbance wavelength shifts from metal nanoparticles, while SPR measures angle changes from a gold film. Both methods yield comparable kinetics and affinity data, but their optical properties have distinct implications.
By design, OpenSPR LSPR systems are:
- Smaller and more affordable, with lower costs for instrument, consumables and service
- Less sensitive to environmental variations like temperature and vibration
- More cost-effective to operate with nanotechnology-based sensors
- Easier to use and maintain with less optical hardware complexity inside
Proven performance with 600+ publications
By detecting biomolecular interactions in real time without additional labeling, OpenSPR assays can reveal which molecules interact, how strongly they interact (affinity), and their rate of interaction (kinetics). OpenSPR affinity and kinetics data has been well-vetted, published by over 600 researchers in high-impact journals worldwide.Add header to the new section: Proven performance with 600+ publications.
Technical specifications
| Performance | |
|---|---|
|
Association Rate (kon) |
1×103–1×107 1/M*s |
|
Dissociation Rate (koff) |
0.1–1×10⁻5 1/s |
|
Affinity Range (KD) |
mM-pM |
| Hardware | ||
|---|---|---|
|
|
OpenSPR |
OpenSPR-XT |
|
Number of Channels |
2 |
2 |
|
Flow Rate |
5–200 µL/min |
5–200 µL/min |
|
Injection |
Semi-Automated |
Fully Automated |
|
Volume per sample |
200 µL |
200 µL |
|
Sample Capacity |
N/A |
2 x 96 well plates |
|
Sample Temperature |
|
Cooled from 22°C–4°C |
|
Unattended Run Time |
|
24 hours |
|
Autosampler |
Optional upgrade to OpenSPR-XT |
Yes |
|
Temperature Range |
4°C–40°C (lower limit 10°C below ambient temperature) |
4°C–40°C (lower limit 10°C below ambient temperature) |
|
Temperature Precision |
+/- 0.25°C |
+/- 0.25°C |
|
Buffer Switching |
3 ports available, automated switching |
3 ports available, automated switching |
|
Instrument Size |
46 x 34 x 21 cm |
78 x 57.5 x 36 cm |
|
Weight |
17 kg |
17 kg (OpenSPR), 21 kg (autosampler) |
|
File Output |
CSV, TXT compatible with TraceDrawer |
CSV, TXT compatible with TraceDrawer |
