Epitope Characterization of Influenza Antiviral Targets Using Alto™


Rapid changes in influenza antiviral target proteins due to antigenic drift result in cloaking of the influenza virus from the immune system of vaccinated hosts. Hence, annual formulation updates for influenza vaccines and related antibody therapies are required to preserve immune recognition against different influenza subtypes. As such, characterizing the binding kinetics and epitope diversity of various antibodies to influenza viral antigens is essential for treating and preventing potential outbreaks. In collaboration with Sino Biological, a global leader in recombinant technology, we use Nicoya’s Alto digital surface plasmon resonance (SPR) system to perform kinetic analysis and epitope characterization of 16 antibodies against Influenza A hemagglutinin (HA), using only 1 µg of antigen and 100 ng of antibody. Alto is now introduced with a simplified 16×16 epitope binning application for biotherapeutics research, galvanizing your productivity by reducing your cost and effort.

Heat map of 16x16 epitope binning from influenza antibodies
Epitope characterization analysis for Influenza A HA antibodies binding to Influenza A H5N1 HA (Cat# 11048-V08H1), sorted by groupings of binders and on-binders, as well as displaying the antibody catalog number. Self blocking is confirmed by the diagonal which shows no