Epitope Characterization of Influenza Targets using OpenSPR™


Rapid changes in influenza antiviral target proteins due to antigenic drift result in cloaking of the influenza virus from the immune system of vaccinated hosts. Hence, annual formulation updates for influenza vaccines and related antibody therapies are required to preserve immune recognition against different influenza subtypes. As such, characterizing the binding kinetics and epitope diversity of various antibodies to influenza viral antigens is essential for treating and preventing potential outbreaks. In collaboration with Sino Biological, a global leader in recombinant technology, we use Nicoya’s surface plasmon resonance (OpenSPRTM) system to perform epitope characterization of several antibodies against Influenza A nucleoprotein (NP).

Epitope characterization analysis for Pan Influenza A Nucleoprotein Antibody binding to Influenza A Nucleoprotein Protein (Cat: 40205-V08B). The degree of binding displayed in the heat map was determined based on the change in response levels after the addition of the solution antibody onto the surface antibody bound to the antigen. Bind, intermediate and not bind categories were defined based on above 30% Rmax, 10-30% Rmax and below 10% Rmax, respectively.