Virus-like particles (VLPs) are complex molecules composed of multiple subunits that resemble a specific virus without its genetic material. VLPs are frequently used to study viruses without being exposed to their infectious components. Their applications include the development of therapies and vaccines against viral diseases, as well as the identification of viral protein components. In this application note, the OpenSPR-XT™ instrument was used to detect a VLP with an approximate molecular weight of 10 MDa to an immobilized antibody. Binding of the VLP could be detected at sub-nanomolar concentrations. The OpenSPR-XT™ is an affordable and user-friendly SPR instrument that enables the measurement of label-free binding kinetics of diverse biomolecular interactions.