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Accelerating insights into changes in binding affinity of biomolecular charge variants using a simple icIEF fractionation and SPR workflow

Details

Charge heterogeneity in monoclonal antibodies can negatively impact binding affinity and potency, so it is critical to ensure that individual charge variants of biotherapeutic molecules are thoroughly characterized to ensure product quality. Dr. Chris Heger (Bio-Techne) and Dr. Mike Piazza (Nicoya Lifesciences) will illustrate a fast, simple icIEF fractionation and digital SPR workflow that provides in-depth characterization of the charge variants of a therapeutic bispecific antibody (BsAb) Mosunetuzumab and a research-grade biosimilar. Using a combination of the MauriceFlex™ system and Alto™ Digital SPR, they demonstrate a novel method that utilizes imaged capillary isoelectric focusing (icIEF)-based charge separation and fractionation followed by binding affinity analysis using surface plasmon resonance, offering a robust and efficient approach to evaluating charge variants. Prior LC-MS analysis of the fractions uncovered interesting differences between the two molecules. The speakers will show how the high-purity fractions of both the innovator drug and a research-grade biosimilar were analyzed by SPR for binding studies with their ligands CD3 and CD20, revealing notable differences in binding affinity, particularly between the biosimilar’s acidic peak and ligand CD20.

Learning Objectives

How to set up a simplified workflow that uses (icIEF)-based charge separation and fractionation followed by digital SPR for deeper insights into charge variant binding affinity.
How leveraging these two benchtop platforms can uncover critical differences between a therapeutic mAb and its biosimilar.
What additional context can be gained on these charge variant differences using mass spectrometry analysis.

Speakers

Dr. Michael Piazza

Director of Applications Development
Nicoya

Dr. Chris Heger

Director, Applications Sciences
Bio-Techne

Resources

Useful resources are linked below.

Brochure: Alto™ digital SPR

As the world’s only surface plasmon resonance (SPR) system powered by digital microfluidics, Alto™ revolutionizes real-time interaction analysis by eliminating the need to compromise on quality and time. Go from sample to answer within hours while streamlining even the toughest of biologics applications with Alto’s intuitive and automated ecosystem. Designed to take the complexity out of SPR, Alto™ will empower your team with the data they need to take their discoveries to the next level.

Read it here.

Nicosystem Software

Alto’s comprehensive software, the Nicosystem, provides a one-stop centralized hub for acquisition and analysis of real-time binding data, all while offering an intuitive user interface, one-click analysis feature, and the flexibility of accessing your experiments from anywhere.

Learn more here.

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Webinars

Accelerating insights into changes in binding affinity of biomolecular charge variants using a simple icIEF fractionation and SPR workflow

Details

Speakers

Dr. Michael Piazza

Director of Applications DevelopmentNicoya

Dr. Chris Heger

Director, Applications SciencesBio-Techne

Resources

Other webinars you might like

Questions or comments about the webinar? Speak with an application scientist today!

Trusted by over 500 research labs worldwide.

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© 2024 Nicoya Lifesciences Inc. All rights reserved. For Research Use Only. Not for use in diagnostic procedures.

Director of Applications Development
Nicoya

Director, Applications Sciences
Bio-Techne

Questions or comments about the webinar?
Speak with an application scientist today!