Overview
The Twin-Strep-Tag® technology from IBA Lifesciences utilizes Strep-Tactin®XT to capture proteins labelled with a Twin-Strep-Tag directionally. Proteintech, a leading provider of antibody reagents, has also developed an anti-Strep VHH that may be used in place of Strep-Tactin XT to capture Twin-Strep-Tags. Both of these capture strategies offer an optimal orientation for analyte binding and enable users to capture ligands from crude samples or matrix compositions, which may be incompatible with direct coupling methods. Protocols for immobilizing StrepTactin XT and Strep VHH have been pre-optimized for Alto™, Nicoya’s Digital surface plasmon resonance™ (SPR) platform, allowing users to reduce experiment design time by offering a pre-developed assay configuration.
Schematic representation of the capture systems used in this study to measure binding between GFP and a HuCAL Fab FH. A) Strep-Tactin XT, from IBA Lifesciences, is immobilized on the carboxyl sensor to capture GFP containing a Twin-Streptag. B) Anti-Strep VHH, from Proteintech, is immobilized on the carboxyl sensor to capture GFP containing a Twin-Strep-tag.