Discover how the Alto™ Digital SPR system can advance your antibody discovery workflows with the Epitope Binning application. Learn how Alto simplifies experiment design and execution by integrating digital microfluidics (DMF) and nanotechnology-based biosensors to automate data collection.
Discover how to efficiently characterize biomolecular interactions with our comprehensive eBook. Learn how the Alto™ digital SPR system simplifies interaction analysis by integrating digital microfluidics (DMF) and nanotechnology-based biosensors for unmatched ease of use and flexibility.
Nicoya’s Protein A Capture Kit allows users to capture antibodies or Fc-tagged proteins directionally, offering an optimal orientation for analyte binding. This method also enables users to capture ligands from crude samples or matrix compositions, which may be incompatible with direct coupling methods. This tech note describes protocols for use of the Protein A Capture Kit with Alto to measure the binding kinetics of Influenza A H5N1 HA antigen and a H5N1 HA antibody.
Nicoya’s Streptavidin Kit allows users to irreversibly capture biotinylated ligands. This method also enables users to capture ligands from crude samples or matrix compositions, which may be incompatible with direct coupling methods. This technical note examines the performance of the Streptavidin Kit with Alto to measure the kinetics of biotinylated IL-6R binding to an anti-IL-6R antibody Tocilizumab, an immunosuppressant.
Nicoya’s Anti-HIS Capture Kit gives users the ability tocapture histidine-tagged ligands directionally, offering an optimal orientation for analyte binding. This method also enables users to capture HIS-tagged ligands from crude samples or from matrix compositions which may be incompatible with direct coupling methods. This technical note examines the performance of the Anti-HIS Capture Kit with Alto to measure the kinetics of HIS-tagged Influenza A H5N1 hemagglutinin (HA) antigen and a H5N1 HA antibody.
Are you considering going label-free for your interaction work? We’ve worked with many researchers over the years to help them succeed with SPR, and we want to help you do the same. To help you get started, we’ve gathered our expertise on this information-rich technique into a handy ebook.