This application note presents an integrated analytical workflow combining an Agilent 1290 Infinity II bio LC system, an Agilent 6545XT AdvanceBio LC/Q-TOF, and a Nicoya Digital Surface Plasmon Resonance (digital SPR) system to provide a holistic assessment of GLP-1 RA structure and function. LC/MS enables detailed structural profiling—including mass confirmation, impurity analysis, and degradation monitoring—while digital SPR delivers real-time, label-free measurements of GLP-1 receptor binding kinetics. When used together, these orthogonal techniques allow developers to directly correlate chemical modifications or degradation products with changes in biological activity. In several cases, this combined approach reveals unexpected disconnects between structural homogeneity and receptor binding behavior, underscoring the value of functional assays alongside structural characterization. By integrating LC/MS and digital SPR, biopharmaceutical scientists can streamline early-stage screening, support stability and forced degradation studies, improve comparability assessments, and gain deeper insight into structure-activity relationships. This workflow aligns with current industry trends toward faster development timelines and more robust characterization strategies, enabling confident decision-making from early discoveries through development and quality control.
