Binding Kinetics of Protein-Protein Interactions using OpenSPR


OpenSPR™ is a powerful instrument providing in-depth label-free binding kinetics for a variety of different molecular interactions. One of the most common applications of surface plasmon resonance is the analysis and quantification of the interactions between proteins. In this application note, OpenSPR™ is used to analyze the kon, koff, and KD of two different protein ligands (M1 and M2) to one protein analyte. The biomolecular system and conditions were developed by Dr. Olan Dolezal from CSIRO.