Overview
Nicoya’s Anti-HIS Capture Kit gives users the ability to capture histidine-tagged (HIS-tagged) ligands directionally, offering an optimal orientation for analyte binding. This method also enables users to capture HIS-tagged ligands from crude samples, or from matrix compositions which may be incompatible with direct coupling methods. This technical note examines the performance of the Anti-HIS Capture Kit with Alto™, Nicoya’s digital surface plasmon resonance™ (SPR) instrument, to measure the kinetics of HIS-tagged Influenza A H5N1 hemagglutinin (HA) antigen and a H5N1 HA antibody.
