Overview
Nicoya’s Streptavidin Kit allows users to irreversibly capture biotinylated ligands directionally, offering an
optimal orientation for analyte binding. This method also enables users to capture ligands from crude samples or matrix compositions, which may be incompatible with direct coupling methods. This technical note examines the performance of the Streptavidin Kit with Alto™, Nicoya’s digital surface plasmon resonance™ (SPR) instrument, to measure the kinetics of biotinylated IL-6R binding to an anti-IL-6R antibody Tocilizumab, an immunosuppressant. Alto’s pre-optimized Streptavidin Kit and protocols allow users to reduce experiment design time by offering a predeveloped assay configuration.
