Overview
Nicoya’s Protein A Capture Kit allows users to capture antibodies or Fc-tagged proteins directionally, offering an optimal orientation for analyte binding. This method also enables users to capture ligands from crude samples or matrix compositions, which may be incompatible with direct coupling methods. This technical note describes protocols for use of the Protein A Capture Kit with Alto™, Nicoya’s digital surface plasmon resonance™ (SPR) instrument, to measure the binding kinetics of Influenza A H5N1 hemagglutinin (HA) antigen and a H5N1 HA antibody. Alto’s pre-optimized Protein A Capture Kit and protocols allow users to reduce experiment design time by offering a pre-developed assay configuration.
