Notes

Analysis of antibody-antigen binding kinetics on Alto™ Digital SPR using a Human/rabbit IgG VHH capture reagent

Overview

Nicoya’s Human/rabbit IgG VHH Capture Kit uses the Nano-CaptureLigand® human IgG/rabbit IgG, Fc-specific VHH (nanobody) from Proteintech to capture human and rabbit antibodies or hu/rb-Fc tagged proteins directionally. This capture strategy offers an optimal orientation for analyte binding and enables users to capture ligands from crude samples or matrix compositions, which may be incompatible with direct coupling methods. The Human/rabbit IgG VHH Capture Kit and protocols are pre-optimized for Alto™, Nicoya’s Digital surface plasmon resonance™ (SPR) platform, allowing users to reduce experiment design time by offering a pre-developed assay configuration.

Figure 1: Schematic representation of an assay using the Human/rabbit IgG VHH Capture Kit.