eBooks & Tech Notes

Tech note: Analysis of antibody-antigen binding kinetics on Alto Digital SPR using a Human/rabbit IgG VHH capture reagent

Nicoya’s Human/rabbit IgG VHH Capture Kit uses the Nano-CaptureLigand® human IgG/rabbit IgG, Fc-specific VHH (nanobody) from Proteintech to capture human and rabbit antibodies or hu/rb-Fc tagged proteins directionally. The Human/rabbit IgG VHH Capture Kit and protocols are pre-optimized for Alto™, Nicoya’s Digital surface plasmon resonance™ (SPR) platform, allowing users to reduce experiment design time by offering a pre-developed assay configuration.

eBook: Epitope Binning

Discover how the Alto™ Digital SPR system can advance your antibody discovery workflows with the Epitope Binning application. Learn how Alto simplifies experiment design and execution by integrating digital microfluidics (DMF) and nanotechnology-based biosensors to automate data collection.

eBook: Mastering kinetic binding assays

Discover how to efficiently characterize biomolecular interactions with our comprehensive eBook. Learn how the Alto™ digital SPR system simplifies interaction analysis by integrating digital microfluidics (DMF) and nanotechnology-based biosensors for unmatched ease of use and flexibility.

Tech note: Analysis of antibody-antigen binding kinetics on Alto Digital SPR using a Protein A capture reagent

Nicoya’s Protein A Capture Kit allows users to capture antibodies or Fc-tagged proteins directionally, offering an optimal orientation for analyte binding. This method also enables users to capture ligands from crude samples or matrix compositions, which may be incompatible with direct coupling methods. This tech note describes protocols for use of the Protein A Capture Kit with Alto to measure the binding kinetics of Influenza A H5N1 HA antigen and a H5N1 HA antibody.

Tech note: Analysis of biotin-tagged proteins on Alto digital SPR using a streptavidin functionalized sensor

Nicoya’s Streptavidin Kit allows users to irreversibly capture biotinylated ligands. This method also enables users to capture ligands from crude samples or matrix compositions, which may be incompatible with direct coupling methods. This technical note examines the performance of the Streptavidin Kit with Alto to measure the kinetics of biotinylated IL-6R binding to an anti-IL-6R antibody Tocilizumab, an immunosuppressant.

Tech note: Analysis of HIS-tagged ligands on Alto digital SPR using an anti-HIS capture sensor

Nicoya’s Anti-HIS Capture Kit gives users the ability tocapture histidine-tagged ligands directionally, offering an optimal orientation for analyte binding. This method also enables users to capture HIS-tagged ligands from crude samples or from matrix compositions which may be incompatible with direct coupling methods. This technical note examines the performance of the Anti-HIS Capture Kit with Alto to measure the kinetics of HIS-tagged Influenza A H5N1 hemagglutinin (HA) antigen and a H5N1 HA antibody.

eBook: A Simple Guide to Surface Plasmon Resonance

Are you considering going label-free for your interaction work? We’ve worked with many researchers over the years to help them succeed with SPR, and we want to help you do the same. To help you get started, we’ve gathered our expertise on this information-rich technique into a handy ebook.

Looking to go label-free for your binding analysis?
Discover the benefits of Surface Plasmon Resonance (SPR)