Application Note
Targeting Optimal Buffers for Downstream Crystallisation Screening
Overview One method of measuring protein stability in a buffer screen is with differential scanning fluorimetry (DSF). Comparison of thermal melting profiles of a protein…
Statistical Repeatability of Tm Values Highlights Utility of Protein Stability Screening Automation
Overview To illustrate the accuracy, and repeatability of microplate-based screening with the SUPR-DSF, 16 x 384-well microplates were prepared with lysozyme using a modular approach…
Formulation screen of Trastuzumab using the SUPR-DSF
Overview In this report we show the analysis of a thermal denaturation-based formulation screen of then commercially available therapeutic antibody Trastuzumab, in 96 different conditions…
Finding Formulations Faster with SUPR-DSF
Overview In this application note we show the analysis of a thermal denaturation-based formulation screen of the commercially available therapeutic antibody Infliximab with the FORMOscreen®…
Speeding Up Early Stage Biotherapeutic Discovery with Next Generation Differential Scanning Fluorimetry
Overview Existing technologies for measuring stability of biologics have all proved useful in identifying candidates that have higher stability than others under certain parameters but…
Analysis of antibody-antigen binding kinetics on Alto Digital SPR using mouse Fc-specific capture reagents
Overview Mouse Fc-specific capture reagents can be used to capture mouse antibodies or Fc-tagged proteins directionally. These capture reagents offer an optimal orientation for analyte…
Analysis of antibody-antigen binding kinetics on Alto Digital SPR using mouse Fc-specific capture reagents
Overview Mouse Fc-specific capture reagents can be used to capture mouse antibodies or Fc-tagged proteins directionally. These capture reagents offer an optimal orientation for analyte…
Temperature dependence of antibody-antigen binding kinetics on Alto Digital SPR
Overview Biomolecular interaction assays are often conducted at subphysiological temperatures due to equipment limitations, however, temperature significantly influences biomolecular behavior, structure, function, and interactions. For…
Kinetic analysis of a recombinant antibody in undiluted serum on Alto Digital SPR
Overview Characterizing the binding and kinetics of antibody antigen interactions in serum is critical for advancing the development of vaccines, diagnostics, and other biotherapeutics. Screening…
Designing label-free biosensor assays for Fc receptor binding analysis: Meeting research challenges
Overview Understanding the binding and kinetics of Fc gamma receptors (FcγR) to monoclonal antibodies is critical for advancing the development of targeted therapies aimed at…
Poster: Rapid Hybridoma Screening with Automated Sample Preparation and Digital SPR Analysis
Overview Alto™ is the worldʼs only Digital SPR™ instrument that uses digital microfluidics (DMF) to handle samples. This allows all SPR sensors, samples, buffers and…
A Novel icIEF Fractionation and SPR-Based Workflow for Correlating the Charge Structure to the Function of a Bispecific Antibody
Overview This application note presents a novel and efficient method for determining binding kinetics and affinity based on the charge heterogeneity of biological molecules. The…
Analysis of HIS-tagged ligands on Alto digital SPR using an anti-HIS capture sensor
Overview Nicoya’s Anti-HIS Capture Kit gives users the ability to capture histidine-tagged (HIS-tagged) ligands directionally, offering an optimal orientation for analyte binding. This method also…
Analysis of biotin-tagged proteins on Alto digital SPR using a streptavidin functionalized sensor
Overview Nicoya’s Streptavidin Kit allows users to irreversibly capture biotinylated ligands directionally, offering anoptimal orientation for analyte binding. This method also enables users to capture…
Analysis of antibody-antigen binding kinetics on Alto digital SPR using a Protein A capture reagent
Overview Nicoya’s Protein A Capture Kit allows users to capture antibodies or Fc-tagged proteins directionally, offering an optimal orientation for analyte binding. This method also…
Poster: A Novel icIEF and Digital SPR Workflow for Correlating the Charge Structure to the Function of a Bispecific Antibody
Overview Biomolecular charge variant characterization has been a challenge that involves a cumbersome and time-consuming process. In this study, we demonstrate the application of the…
The dynamic workflow of Alto™ digital SPR for Influenza vaccine development
Overview Rapid changes in influenza antiviral target proteins due to antigenic drift result in the cloaking of the influenza virus from the immune system of…
Poster: Identification of Anti-Idiotype Antibodies using Digital SPR
Overview Anti-idiotypic antibodies (anti-IDs) recognize the unique variable region of another antibody called the idiotype (defined below). Anti-IDs are commonly used in bioanalytical assays to…
