Overview
Understanding the binding and kinetics of Fc gamma receptors (FcγR) to monoclonal antibodies is critical for advancing the development of targeted therapies aimed at modulating immune responses effectively. These interactions can greatly impact therapeutics safety and efficacy, making efforts to analyze and enhance Fc interactions with FcγRs an important aspect of the development of biotherapeutics.
When analyzing Fc receptor binding using label-free biosensors, the complexity involved in assay development and optimization places hurdles in accessing high-quality data. Often, a high level of expertise is needed to develop optimal assay methods, high volume of expensive antigens may be needed, and time to results can be long.
In this application note, we demonstrate how the Alto™ Digital SPR™ system offers a superior innovation in label-free biosensors for the measurement of binding affinities and kinetics of antibodies to different classes of FcγRs, highlight best practices for various assay formats and capture surfaces, and describe assay optimization and data analysis.
