Overview
Capture coupling techniques are advantageous to investigate biomolecular interactions as they provide specificity for immobilization on the OpenSPR real-time, label-free biosensor platform. Streptavidin sensors offer a streptavidin coated sensor surface which can capture biotin-tagged ligands with high affinity. However, the biotin-streptavidin interaction is irreversible, limiting researchers to one biotinylated ligand per sensor. In this application note, Eliza Hanson, a member of Rebecca Whelan’s research team at the University of Kansas explored the use of His-streptavidin on OpenSPR NTA sensors to create a regeneratable surface and measure binding interactions for multiple biotinylated aptamer ligands on a single sensor.
