Binding Kinetics of Glycoprotein Interactions using OpenSPR

Overview

OpenSPR™ is a powerful instrument providing in-depth label-free binding kinetics for a variety of different molecular interactions. One of the most common applications of surface plasmon resonance is the analysis and quantification of the interactions between proteins and other molecules. In this application note, OpenSPR™ is used to analyze the kon, koff, and KD of a glycan – concanavalin A (Con A) interaction. Con A is a lectin (carbohydrate binding protein) that binds to a number of sugars, glycans, glycoproteins, and glycolipids.