Secondary Structure: What is it?
For many decades, the most prevalent application of Circular Dichroism spectroscopy has been the quantification of secondary structure in polypeptides. To this end, far-UV CD data are subjected to secondary structure decomposition analysis, which yields estimates for the fractional amounts of different secondary structure elements in the peptide or protein of interest. Usually, the results are expressed in percentages and always include at least numbers for helices, sheets, turns, and random coil secondary structure. But what exactly are these?
Explore a gallery of more than five dozen protein structures below to learn about secondary structure elements, and create a small peptide in our playground further below to gain a better understanding of the underlying principles of secondary structure classification.
Drag the left mouse button to rotate 3D views and drag the middle mouse button or use the mouse wheel to zoom. After spending some time on this page, you will be able to appreciate that secondary structure is not just about α-helices and ß-sheets!
Note: Some of the content below is simplified and goes without claim to be a replacement for existing literature. Please see the references provided for more detailed information.
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Explore Secondary Structure
Defining Secondary Structure
When we talk about the secondary structure of a polypeptide, we basically refer to the local three-dimensional arrangement of its amino acid main chain, or backbone, into a series of linear segments that are stabilised by hydrogen bonds in well-defined, repeating patterns.
Firstly, secondary structure can be defined based on gemometric criteria for the hydrogen bonds which are formed between amide (N-H) and carbonyl (C=O) groups in those segments: Typically, these criteria are as follows (Taylor et al. 1984):
- A H···O distance < 2.5 Å
- A N···O distance < 3.5 Å
- A N-H···O angle > 110°
Secondly, secondary structure can be defined based on the more or less specific relative positions of backbone atoms. These can be described by means of torsion angles, i.e., dihedral angles in a stereochemical context, where both terms are usually being used synonymously.
Torsion Angles
A dihedral angle, in general, is the angle between two intersecting planes or half-planes, and a torsion angle, in particular, is the dihedral angle between two planes on either side of a chemical bond that each span across a set of three atoms, where both planes include the two atoms that form the bond as well as the atom before or after this pair of atoms. Upon rotating both ends on either side of a bond in a molecule against each other, the torsion angle for that bond changes.
In proteins and peptides, three types of torsion angles can be distinguished for:
- The bond preceding a Cα atom, φ (phi): Ci – 1—Ni—Cαi—Ci
- The bond following a Cα atom, ψ (psi): Ni—Cαi—Ci—Ni + 1
- The peptide bond, ω (omega): Cαi—Ci—Ni + 1—Cαi + 1
- where i depicts the number of a residue (and i + 1 is the subsequent residue in the amino acid sequence), the two planes span the first three atoms and the last three atoms, respectively, and rotation around the bond in the middle affects the corresponding torsion angle.
The peptide bonds in polypeptides have a partial double-bond character due to the delocalisation of the lone pair of electrons on the nitrogen atom. Therefore, peptide bonds almost exclusively assume a trans configuration, and the corresponding dihedral angle, ω, is virtually always close to 180°.
As opposed to the torsion angle of the peptide bond, φ and ψ have more degrees of freedom. However, G. N. Ramachandran showed in 1963 that not just any theoretically possible combination of φ and ψ is found in proteins, but rather dihedral angles are subject to certain constraints (which were later refined, e.g., see Lovell et al. 2003) and can be classified accordingly:
- Allowed combinations that won’t result in steric clashes between atoms
- Favoured combinations that allow for the formation of stabilising backbone hydrogen bonds
These constraints are the reason why secondary structure can be classified into certain reoccuring elements that are characterised by sets of specific (within margins) dihedral angles and give rise to specific patterns of hydrogen bonding.
