Presenters:
Michael Piazza, PhD
AVP, Applications Development
Nicoya Lifesciences
Alyssa Ball, PhD
Professor, Assay Development Scientist
Conigen Bioscience
Overview
Understanding cytokine-receptor interactions is critical for advancing immunology research and therapeutic development, but capturing these interactions in a native-like state can be challenging.
In this webinar, Conigen Bioscience’s Assay Development Scientist, Dr. Alyssa Ball will share how they leveraged Nicoya’s Alto digital surface plasmon resonance (SPR) system to characterize the binding kinetics of several homo- and heterodimeric cytokine receptors, including human Type II interferon γ receptor 1 (IFNγR1) and human Type I interferon α receptor (IFNαR).
By mimicking the natural dimeric conformations of cytokine receptors, the team achieved significantly enhanced binding potency to their respective cytokines (IFNγ and IFNα) – insights made possible by Alto’s real-time, label-free analysis in a high-throughput, digital format.
Topics covered in this webinar:
- How Conigen Bioscience’s innovative soluble protein dimers enhance ligand binding sensitivity and potency of cytokine receptor proteins
- Why SPR was critical for revealing nuanced differences in binding potency
- How Alto digital SPR enables rapid, high-quality kinetic data for complex receptor-ligand systems
- How real-time, label-free kinetic analysis combined with structural and stability characterization can accelerate the selection of functionally relevant and stable therapeutic candidates
