Access: Granted. Deepen discovery with OpenSPR
Get the publication-quality binding kinetics and affinity data you’ve been missing, without breaking your budget.
Open your lab to uncompromised SPR
OpenSPR offers entry-level, benchtop SPR at a fraction of the cost of competitors, without compromising data quality.
Nicoya’s OpenSPR® platform is a 2-channel instrument, developed to reduce the cost and complexity of SPR assays, while allowing more labs access to kinetic insights. Utilizing our proprietary localized SPR (LSPR) technology, the OpenSPR platform provides accurate and robust data, all from your lab bench.
Bring SPR to your benchtop
By design, our streamlined LSPR technology packs high-quality interaction analysis into a small benchtop footprint. This lets you reclaim lab space and gives your team room to grow, adapt, and scale future research.
Label-free analysis at a fraction of the cost
OpenSPR utilizes nanosensor technology to keep instrument, consumable, and service costs low. This allows your lab to do more with less.
Keep your students ahead of the game
OpenSPR provides students with hands-on access to a vital, widely-used technique shaping the fields of drug discovery, biochemistry, and medical diagnostics. Training your students on OpenSPR helps prepare them to start their careers or continue in their research.
Maximize your throughput with OpenSPR-XT
Add on the XT Autosampler to increase OpenSPR’s throughput without purchasing a new instrument. This tool automates OpenSPR workflows, allowing for walk-away operation, increased overall sample capacity—and more data with less hands-on time.
Supported Assays/Compatible Molecules
Supported Assays
The OpenSPR platform is designed to support a variety of assay types, including:
- Kinetics/affinity characterization
- Kinetics/affinity screening (upgrade to OpenSPR-XT recommended)
- Yes/no binding
- Concentration analysis
- Competition assays
- Epitope mapping
Compatible Molecules
OpenSPR is compatible with a wide range of molecules, including:
- Proteins
- Peptides
- Antibodies + antibody variants
- Nucleic acids
- Lipids
- Adeno-associated viruses (AAVs)
- Virus-like particles (VLPs)
- Hormones/cytokines
- Small molecules (application dependent)
LSPR – The core of OpenSPR
Localized surface plasmon resonance (LSPR) is a variation of traditional SPR. Instead of using a continuous gold film, LSPR uses metal nanoparticles (typically gold or silver). These nanoparticles produce a strong absorbance peak in the range of visible light, and are highly sensitive to the local refractive index surrounding the particle. OpenSPR’s advanced spectrometer measures small changes in the nanoparticles’ absorbance wavelength, before translating them into binding events.
LSPR has a number of significant advantages, including:
- Simpler optical hardware that allows for a smaller, sturdier and more affordable instrument.
- Since measuring a reflected light angle is not important, the instrument is much more robust against vibration and mechanical noise
- No strict temperature control simplifies operation.
- Sensor chips that can be manufactured at a much more affordable price
- Ease of use and maintenance
Product Specifications
Technical Overview
| Category | OpenSPR Specification | OpenSPR-XT Specification |
|---|---|---|
| Throughput | 2 channels | 2 channels |
| Sample Volume | Injection volume + 50 μL | 200 μL |
| Cartridge Types | Multiple surface chemistries | Multiple surface chemistries |
| Technology | LSPR | LSPR |
| Automation | Semi-automated injections | Automated injections |
| Maintenance | Some required | Some required |
| Footprint | Benchtop system – 0.67H x 1.5W x 1.1D (ft) | Benchtop system – 1.2H x 2.5W x 1.9D (ft) |
| Affinity Range | pM – mM | pM – mM |
| Applications | Kinetics & affinity, yes/no binding, concentration analysis, competition assays, epitope mapping | Kinetics & affinity, screening, yes/no binding, concentration analysis, competition assays, epitope mapping |
OpenSPR Software Suite
The OpenSPR software suite is designed exclusively for our OpenSPR instrument. It provides researchers with guided setups, ligand immobilization wizards, exports for quick and easy data analysis, and more. The software suite is included with the purchase of an OpenSPR Starter Kit.
Affordable sensors
Our nanotechnology-enabled sensors are manufactured to ensure consistent and repeatable measurements at half the price of traditional sensors. In addition to a wide range of sensor chemistries available, we also offer scientists the option for high-sensitivity sensors to enhance biomolecular signals or analyze small molecules. All of our available sensors can be purchased through our online store.
Resources
Analysis of antibody-antigen binding kinetics on Alto Digital SPR using mouse Fc-specific capture reagents
Overview Mouse Fc-specific capture reagents can be used to capture mouse antibodies or Fc-tagged proteins directionally. These capture reagents offer an optimal orientation for analyte…
Nicoya Alto CBX Cartridge Sensor Performance
With Alto, Nicoya’s Digital surface plasmon resonance™ (SPR) platform, the entirety of the fluidic control and sensing is done on single-use disposable cartridges. High accuracy…
Uncovering New Tools to Analyze Cytokine–Receptor Interactions: How Conigen Bioscience Used Nicoya’s Alto™ Digital SPR to Characterize Novel Dimeric Receptors
Presenters: Michael Piazza, PhD AVP, Applications Development Nicoya Lifesciences Alyssa Ball, PhD Professor, Assay Development Scientist Conigen Bioscience Overview Understanding cytokine-receptor interactions is critical for…
Open your lab doors to affordable SPR
Talk to our team to see how you can get the publication-quality interaction data you’ve been missing with OpenSPR.
