Overview
Precise and accurate liquid handling is crucial to obtaining repeatable, high-quality data with any analytical instrument. This is especially true when preparing samples for surface plasmon resonance (SPR) experiments, since the sample concentration is the only known variable when solving for kinetic constants. Without accurate and precise liquid handling, error rapidly creeps into concentration calculations, and consequently into kinetic fits. From this study, we conclude that Alto is more precise than the majority of pipettes and liquid handlers, when operating at similar volumes. We also demonstrate that compared to those prepared with a mechanical pipette, Alto-made serial dilutions are more than twice as accurate after three dilutions.
