Notes

Binding Kinetics of Glutathione-S-Transferase (GST) Tagged Proteins Using OpenSPR

Overview

OpenSPR™ is a powerful instrument providing in-depth label-free binding kinetics for a variety of different molecular interactions. One of the most common tags used for protein separation is glutathione-Stransferase (GST). Proteins containing this tag bind freely to glutathione. Nicoya Lifesciences has created a glutathione functionalized sensor that makes immobilization of GST tagged proteins simple. In this application note, OpenSPR™ is used to analyze the KD of the GST-Ubiquitin with Anti-Ubiquitin Antibody.