Notes

Binding Kinetics of Carbonic Anhydrase II to Small Molecules Using OpenSPR High Sensitivity Sensors

Overview

OpenSPR™ is a powerful instrument providing in-depth label-free binding kinetics for a variety of different molecular interactions. One of the most interesting areas of study are small molecule–protein interactions. OpenSPR™ High Sensitivity Carboxyl Sensors were used to measure kinetic binding between Carbonic Anhydrase II (CAII), a thoroughly studied small molecule binding protein, to furosemide and acetazolamide, small molecules with molecular weights of 331 Da and 222 Da respectively.