Protein Binding Kinetics

Label-Free Protein Analysis

Benchtop SPR is a user-friendly tool that allows researchers to characterize biomolecular interactions in real-time measurements. Analyze the kon, koff, and KD of your protein interaction with Nicoya's OpenSPR. 


  • Determine kon, koff, and KD values (Affinity)
  • Quantify levels of protein at different development stages
  • Quantity levels of protein at different expression levels
  • Yes/No binding
  • Determine presence of protein and antibody
  • Determine levels of crude antibody titer
  • Screening for ligand binding partners
  • Specificity

Application Highlight

Kinetic analysis of a protein-protein interaction using OpenSPR.

Use Cases

  • Expressed protein interactions
  • Extracted protein interactions
  • Crude lysate binding detection and relative quantification
  • Antibodies
  • Quality control
  • Drug discovery

"The OpenSPR is a very versatile and affordable system that has allowed us to accurately measure protein-protein and protein-peptide interactions without the need to label the interacting molecules. It is user-friendly, versatile and the sensor chips are not very expensive. Both graduate and undergraduate students have used the instrument to generate reliable data."

Dr. Guy Guillemette

Chemistry, University of Waterloo

Book 1-on-1 Web Demo

Book a free live web demo with one of our application scientists to discuss your research applications and learn how OpenSPR works step-by-step.