Nucleic Acid Binding Kinetics

Label-Free Nucleic Acid Analysis

Analyze protein-DNA, protein-RNA and aptamer interactions with your personal SPR. Compact, user-friendly and affordable, Nicoya’s OpenSPR allows for label-free analysis on your own bench. Forget radiolabeling and fluorescent labels! Determine nucleic acid kinetics and affinity constants (kon, koff, KD), presence of a protein/binding events or quantify analyte levels in no time.

Outcomes

  • Determine kinetics (kon, koff) and affinity (KD) values
  • Quantify levels of protein at different development stages
  • Yes/No binding and screening of SELEX aptamers
  • Screening for ligand binding partners
  • Specificity

Application Highlight

Aptamer-protein interaction analyzed with OpenSPR to determine binding kinetics

Use Cases

  • Aptamer characterization
  • Analyzing DNA and RNA interactions
  • Expressed protein interactions
  • Extracted protein interactions
  • Aptamer quality control
  • Drug discovery
  • Therapeutic and diagnostic applications
  • Aptamer biosensor development

"We have used label-free analysis tools in the past, but they are expensive to purchase and maintain and have steep learning curves. OpenSPR offers the perfect combination of performance and affordability, which will accelerate our research while saving our lab money. At the same time the system is very flexible and allows for a wide variety of experiment designs and applications"

Dr. Thorsten Dieckmann

Chemistry, University of Waterloo

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